Skip to main content
. 2016 Dec 23;6(2):296–304. doi: 10.1242/bio.018226

Fig. 3.

Fig. 3.

GFP-ODD responds to modulation of HIF pathway components. Levels of GFP-ODD fluorescence (green) were analysed in wing imaginal discs of third-instar larvae, in which regulators of the hypoxia response were either depleted by RNAi or overexpressed in the posterior compartment using the en-Gal4 driver. Cells in the posterior compartment (marked ‘P’ in panel A″) are labelled by the en-Gal4-driven expression of UAS-mCherry-nls (A, magenta in A″). The anterior compartment (marked ‘A’ in panel A″) serves as an internal control. In control larvae (lacZ-RNAi; A-A″), GFP-ODD levels in the anterior and posterior compartment are indistinguishable. RNAi-mediated knockdown of dVHL (B-B″) or fatiga (C-C″) in the posterior compartment results in accumulation of GFP-ODD. Conversely, overexpression of Fatiga-A leads to lower levels of GFP-ODD (D-D″). (E-E″) Depletion of PTEN in the posterior compartment results in accumulation of GFP-ODD. (F-F″) Inhibition of the mitochondrial respiratory chain through COXVb RNAi causes accumulation of GFP-ODD. Scale bar: 100 µm. (G) Bar graph showing ratios between mean GFP-ODD intensities in the posterior (GFP[P]) and anterior compartment (GFP[A]). Ratios were normalised to the values of the RNAi control (en>lacZ RNAi). Bars indicate mean values, error bars represent the standard deviation. The number (n) of imaginal discs analysed for each genotype is indicated.