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. 2017 Feb 8;6(2):165–175. doi: 10.1242/bio.020040

Fig. 4.

Fig. 4.

Postembryonic expression of Crb isoforms. (A-D) Expression of Crb isoforms in tissues of wild-type third instar larvae stained with α-Crb_C (green), α-Crb_Ex3 (magenta), and α-Stranded at Second (SAS, cyan) as apical marker. Crb localises to the apical membrane. In the eye disc, Crb is predominantly expressed behind the morphogenetic furrow, and is strongly enriched at the apical membranes of differentiating photoreceptor cells (arrow in C). Crb_C expression is not detected in the examined imaginal discs (A-C), but is observed in the larval salivary gland (D). Scale bars: 50 µm. (E) Gel electrophoresis of RT-PCR experiments to identify transcribed crb mRNAs. Larval, RNA from larval large intestine; adult, RNA from adult flies; H2O, negative control (no DNA). For primers used, see Table S2. M: 1 kb DNA Ladder. 1, 2, 3: primer pair 1, expected sizes crb-RA 919 bp, crb-RB/D 1048 bp, crb-RC 1240 bp. 4, 5, 6: primer pair 2, expected sizes crb-RA 1285 bp, crb-RB/D 1414 bp, crb-RC 1606 bp.