Fig. 6.

crb_C mutant photoreceptor cells exhibit normal morphology. (A-J) Representative TEM images of retinal cross sections (A,C,E,G,I) and confocal images of longitudinal retinal sections (B,D,F,H,J) stained for Chaoptin (green) and Phalloidin (red) of adult flies, prepared 2 days after eclosion (light/dark cycle). crb^11A22 show mosaic eyes, all other eyes were from flies of the indicated allele/allelic combination. Fused rhabdomeres (red arrow) and incompletely elongated rhabdomeres (white arrows) are only seen in crb^11A22 mosaic retinas. Scale bars: 1.7 µm (A,C,E,G,I), 50 µm (B,D,F,H,J). (K) Mean stalk length (nm)±s.e.m. of PRCs of different genotypes. Statistically significant changes between genotypes (highlighted by a black line) are indicated with ***P<0.05E-10 and *P<0.05E-4 following ANOVA and post hoc Bonferroni test. The average reduction in stalk length is 20.7% for crb^p17F5, 14.5% for crb^p13A9/crb^11A22, 9.9% for crb^p13A9, and 41% for crb^11A22 with respect to the genetic control (w*).