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. 2016 Dec 23;6(2):148–160. doi: 10.1242/bio.021063

Fig. 6.

Fig. 6.

Raldh2cKO does not affect cell cycle exit of NPCs. (A-D′) Immunolabellings on brain sections from E14.5 control and Raldh2cKO animals analysed for BrdU (green), which was injected 24 h prior to analysis as a single pulse, and Ki67 (red) at rostral (A-D) and more caudal levels (A′-D′) used for quantification. (E-H′) Immunolabellings on brain sections from E16.5 control and Raldh2cKO animals similarly analysed for BrdU (green) injected 24 h prior to analysis and Ki67 (red). The proportion of cells leaving the cell cycle (BrdU+/Ki67– over BrdU+ cells) is unchanged in Raldh2cKO mutants versus controls. (I) Quantifications at E14.5; rostrally: 25.58±0.54 for control and 27.57±0.75 for Raldh2cKO; caudally: 27.53±0.75 for control and 29.83±1.25 for Raldh2cKO. (J) Quantifications at E16.5; rostrally: 33.92±0.84 for control and 29.92±1.92 for Raldh2cKO; caudally: 31.90±0.74 for control and 31.69±0.92 for Raldh2cKO. Data presented as mean±s.e.m.; n=5 brains; ns, not significant by two-tailed Student's t-test. Scale bars: 50 μm.