Skip to main content
. 2017 Jan 9;6(2):252–259. doi: 10.1242/bio.021774

Fig. 1.

Fig. 1.

miR-142 is largely downregulated in both pancreatic cancer tissues and cell lines and hinders the proliferation and invasion of PANC-1 and SW1990 cells. (A) The expression of miR-142 was determined using SYBR green quantitative PCR in pancreatic cancer tissues compared to the adjacent normal tissues in a panel of matched tissues from 42 pancreatic cancer patients. (B) The miR-142 expression was determined in four pancreatic cancer cell lines compared with human pancreas cell line, HPC-Y5 using real-time PCR. (C) PANC-1 and SW1990 cell line were transfected with miR-142 mimics or miR-142 inhibitor, respectively, and the transfection efficiency was verified using real-time PCR. (D,E) The proliferation of PANC-1 and SW1990 cells in response to miR-142 overexpression or inhibition was determined using MTT assay. (F,G) The invasion of PANC-1 and SW1990 cells in response to miR-142 overexpression or inhibition was determined using Transwell assay. **P<0.01; one-way ANOVA. The data are showed as mean±s.d. of three independent experiments.