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. 2016 Nov 28;6(2):133–140. doi: 10.1242/bio.022483

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Characterization of the ex vivo 3D tumor model. (A) Scanning electron microscopy (SEM) analysis of collagen-based scaffolds at different magnifications. Images were taken with a FEI Nova NanoSEM microscope. (B) Pictures of collagen scaffolds pre- and post-cellularization with primary liposarcoma cells and hematoxylin & eosin staining of paraffin-embedded sections of the scaffold pre- and post-cellularization (C) Hematoxylin & eosin staining of paraffin-embedded sections of 3D scaffolds cultured with primary liposarcoma cells and of the histological specimen. Arrowheads indicate tumor cells. (D) Inverted microscopy pictures of 2D-cultured and 3D-recovered liposarcoma cells.