FIG 8.

Coordination of replication between the two chromosomes of V. cholerae. A novel site in Chr1 (crtS [marked in green]) binds and remodels RctB such that its binding to 12-mers increases and its binding to 39-mers decreases (62). Since 12-mer binding helps to activate initiation and 39-mer binding helps to inhibit it, the changes in both kinds of binding by crtS are favorable to Chr2 replication. The position of the site (around 3 o'clock) is such that a replication fork would travel across it shortly before the time of Chr2 replication initiation. The timing of Chr2 replication is thus made dependent on the position of the crtS site (63).