Figure 1.

HuCNS-SC RCLs Exhibit Engraftment and Improve Locomotor Recovery in a 9 DPI Transplantation Paradigm

(A) Representative images from cervical SCI groups receiving HuCNS-SC RCL transplant (left) or vehicle control (right) 9 DPI. Animals were sacrificed 12 WPT. Sections were immunostained with a human-specific cytoplasmic marker STEM121 (brown) and counterstained with methyl green.

(B) Blinded, unbiased stereological quantification 12 WPT revealed an average of 109,695 ± 25,197 human cells in the RCL group (n = 9). Dashed line denotes transplant dose of 75,000 cells.

(C and D) Animals receiving RCL 9 DPI (n = 12) exhibited a significant decrease in horizontal ladder beam errors at 12 WPT for both ipsilateral (13.4 ± 3.0 versus 24.5 ± 5.5 for controls) and contralateral (0.2 ± 0.1 versus 3.8 ± 1.4 for controls) forepaws (Student's one-tailed t test, ^∗p < 0.05) compared with controls (n = 13 ipsilateral, 12 contralateral). Data shown as means ± SEM.