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. 2017 Feb 14;8(2):249–263. doi: 10.1016/j.stemcr.2016.12.018

Figure 2.

Figure 2

HuCNS-SC CCLs Exhibit Reduced Engraftment Compared with RCLs in a 60 DPI Paradigm

(A) Representative images from cervical SCI groups that received either a CCL, RCL, or hFb transplant, or vehicle control 60 DPI. Animals were sacrificed 12 WPT. Sections were immunostained with STEM121 (brown) and counterstained with hematoxylin (purple).

(B) Blinded quantification 12 WPT revealed an average of 91,701 human cells for the CCL group (n = 16), 200,754 for RCL group (n = 17), and no surviving human cells for the hFB group (n = 12) A one-way ANOVA revealed significant differences in survival (solid bar p < 0.0001; post hoc Tukey's test of CCL versus RCL and hFb, ∗∗∗∗p < 0.0001). Dashed line denotes transplant dose of 75,000 cells.

(C) No difference was found in the rostral-caudal extent of human cell migration between the CCL (n = 16) and RCL (n = 17) at 12 WPT (unpaired t tests with Holm-Sidak multiple comparison correction, p > 0.05). Dashed vertical line indicates injury epicenter. Data shown as means ± SEM.