Figure 4.

Analysis of Locomotor Recovery in HuCNS-SC 60 DPI Cohort Transplantation Groups versus Vehicle Controls 12 WPT

(A) Table showing average ± SEM values for cylinder reaching (percentage paw placement), grip strength, horizontal ladder beam errors, and CatWalk step kinematic analysis for each experimental group. RF, ipsilateral forelimb; LF, contralateral forelimb. Where one-way ANOVA reached significance (^∗p ≤ 0.05, ^∗∗p ≤ 0.01), Tukey's multiple comparison t tests were conducted. Significant differences were found in ipsilateral cylinder reaching between the hFB and Injured only groups (^∗p ≤ 0.05), and in Aa step pattern between the RCL, CCL, and hFB groups (^∗p ≤ 0.05). n = 15–16 for CCL, n = 16–17 for RCL, n = 11–12 for hFB, n = 15–17 for Vehicle, and n = 9–10 for Injured only (see Figure S1D for exact numbers). BOS, base of support.

(B–G) Pearson correlations were conducted between the number of human STEM121⁺ cells and (B) ipsilateral forelimb ladder beam errors, (C) Ab step pattern, and (D) Ca step pattern. Pearson correlation were also conducted between STEM121⁺/CC1⁺ human oligodendroglial cell proportion and (E) ipsilateral forelimb ladder beam errors, (F) Ab step pattern, and (G) Ca step pattern. Dashed lines indicate confidence intervals of 95%. n = 7 for CCL and n = 7 for RCL in CC1+ correlational analyses.