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. 2017 Feb 14;8(2):249–263. doi: 10.1016/j.stemcr.2016.12.018

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Lineage Analysis of HuCNS-SC CCL in the 9 DPI Paradigm

Data for proportional human cell fate were collected by blinded, unbiased stereology

(A) Immunostaining for STEM121 (brown) and DCX (blue) revealed no STEM121⁺/DCX⁺ cells in CCL 9 DPI animals. Inset shows positive control for DCX in cortex.

(B) Immunostaining for human-specific GFAP (STEM123, brown) with hematoxylin counterstaining (purple) revealed 33% ± 4.4% of CCL 9 DPI were positive for this astroglial marker.

(C) Immunostaining for STEM121 (blue) and Olig2 (brown) revealed that the largest proportion of STEM121⁺ cells in the 9 DPI transplant group were positive for this oligodendroglial marker (57.6% ± 2.4%).

(D) Immunostaining for STEM121 (brown) and CC1 (blue) revealed 13.6% ± 3% of CCL 9 DPI were positive for this mature oligodendroglial marker.

Arrowheads indicate double-positive cells. n = 7 for all lineage tests. Data shown as means ± SEM.