Figure 7.

Locomotor Recovery in HuCNS-SC 9 DPI Cohort Transplantation Groups versus Vehicle Controls 12 WPT

Data for proportional human cell fate were collected by blinded, unbiased stereology.

(A) Average ± SEM values for cylinder reaching (percentage paw placement), grip strength, horizontal ladder beam errors, and CatWalk kinematic analysis for each group. RF, ipsilateral forelimb; LF, contralateral forelimb. No significant differences were found between groups using one-way ANOVA p = 0.06; however Tukey's multiple comparison revealed significant an increase in ipsilateral forelimb ladder beam errors in the hFB group compared with vehicle group (p < 0.05) and in contralateral forelimb ladder beam errors in CCL 9 DPI group compared with injured only group (p < 0.05), denoted by ^∗. n = 18–19 for CCL, n = 17–18 for hFB, n = 18–20 for Vehicle, and n = 9–10 for Injured only (see Figure S1D for exact numbers).

(B–G) Pearson correlations were conducted between the number of human STEM121⁺ cells and (B) ipsilateral forelimb ladder beam error number, (C) Ab step pattern, and (D) Ca step pattern (n = 18). Pearson correlations were also run between STEM121⁺/CC1⁺ human oligodendroglial cell proportion and (E) ipsilateral forelimb ladder beam errors, (F) Ab step pattern, and (G) Ca step pattern (n = 7). No significant differences were observed, suggesting neither improvements nor decrements in function at 9 DPI. Dashed lines indicate confidence intervals of 95%.