Figure 1.

C/EBPα, β, δ, and ε Transdifferentiate B Cells to Myeloid Cells

(A) pMSCV-based vectors containing an internal ribosomal entry site (IRES) and EGFP (MIG) marker used for retroviral transduction. Full-length open reading frames of Cebpa, Cebpb, Cebpd, or Cebpe were inserted upstream of the IRES element. Empty vector (MIG) served as control.

(B) Flow cytometric analysis of GFP⁺-infected B cells 4 days after transduction with individual C/EBPs.

(C) Analysis of granulocyte (Ly-6G) and macrophage (CD115) cell-surface markers in GFP⁺CD11b⁺ transdifferentiated cells 6 days after transduction.

(D) May-Grünwald staining of GFP⁺-sorted B cells 4 days after transduction. Arrows indicate cells with typical macrophage morphology and arrowheads mark granulocyte-like cells. Primary bone marrow (BM) and sorted granulocytes (Gr) were used as references (far right).

See also Figure S1.