Figure 4.

C/EBPβ Transgene Dosage Affects Macrophage versus Granulocyte Lineage Outcome

(A) Data binning of C/EBPβ-transduced B cells according to GFP intensity (GFP1–6).

(B) Flow cytometric analysis of GFP1–6 bins (CD11b + gated) 6 days after transduction.

(C) Quantification from five independent experiments. Data are presented as mean ± SEM.

(D) Cytospin preparation and May-Grünwald staining of GFP^dim- and GFP^high-sorted C/EBPβ-transduced B cells at day 7. Arrows indicate macrophage and arrowheads granulocyte-like cells.

(E) B cells were transduced with C/EBPε, sorted after 24 hr as GFP⁺ or GFP^high, and recultured. Flow cytometric analysis of granulocyte (Ly-6G⁺) versus macrophage (CD115⁺) markers in GFP⁺CD11b⁺-gated populations 6 days post sorting.

See also Figure S3.