Figure 2.

TERC Expression Increases during In Vivo Reprogramming

(A) Representative images corresponding to TERC detection by RNA-FISH (red) followed by immunostaining to detect OCT4 (green) in the pancreas of induced reprogrammable mice of the indicated genotypes. Arrowheads indicate the presence of TERC foci. Scale bars, 25 μm. Zoomed areas (orange boxes) of TERC detection are shown on the right. Scale bars, 50 μm. White dashed lines contain OCT4-positive regions.

(B) Number of TERC spots per nuclei. Expression of TERC is increased in the reprogrammed areas. Error bars denote SE. Statistical analysis by Student's t test. Number of mice analyzed is indicated.

(C) Expression of TERC, TRF1, and OCT4 in non-reprogrammed pancreas compared with in vivo iPSCs of pancreatic origin as measured by qRT-PCR. Expression of these genes is strongly upregulated during the acquisition of pluripotency in vivo. Error bars denote SE. Statistical analysis by Student's t test. n, number of independent pancreas or in vivo iPSC clones obtained from independent reprogrammed pancreas, respectively.

See also Figures S1 and S2.