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. Author manuscript; available in PMC: 2017 Feb 16.
Published in final edited form as: Cell Rep. 2016 Dec 20;17(12):3233–3245. doi: 10.1016/j.celrep.2016.11.068

Figure 6. DA Neurons Lacking TβRII Showed Excitation-Inhibition Imbalance and Reduced Synaptic Stimulation-Induced Burst Firing.

Figure 6

(A and B) Traces of mEPSC (A) and mIPSC (B) recorded from DA neurons from littermate control (top) and DAT-iCre;Ai14;TbRIIfl/fl mutants (bottom).

(C) Cumulative probability plots of mEPSC inter-event intervals (left, *p < 0.05, Kolmogorov-Smirnov [KS] test) and summary of mean mEPSC frequency (right, p > 0.21, Mann-Whitney). Number of neuron recorded, n = 16 from three DAT-iCre;Ai14;TbRII+/+ mice and n = 17 from four DAT-iCre;Ai14;TbRIIfl/fl mice.

(D) Cumulative probability plots of mIPSC inter-event intervals (left, p < 0.05, K.S test) and mean mIPSC frequency (right, p < 0.05, Mann-Whitney). Number of neuron recorded, DAT-iCre;Ai14;TbRII+/+: n = 16; DAT-iCre;Ai14;TbRIIfl/fl: n = 17.

(E–G) Evoked EPSCs (left) and input/output curve for evoked EPSC (right) (E), IPSCs (left) and input/output curve for evoked IPSC (right) (F), and excitation/inhibition ratios (EPSC/IPSC ratios) (G). Number of neuron recorded, DAT-iCre;Ai14;TbRII+/+: n = 16 and DAT-iCre;Ai14;TbRIIfl/fl: n = 17. *p < 0.05, Mann-Whitney.

(H) Spontaneous and burst firing of DA neurons recorded from littermate control (left) and DAT-iCre;Ai14;TbRIIfl/fl conditional knockout mice (right) in response to high-frequency stimulation. Rectangular bar indicates stimulation (100 Hz, 1 s).

(I) Number of AP of burst firing, maximal frequency of burst firing, and mean frequency of burst firing evoked by high-frequency stimulation (*p < 0.001, Mann-Whitney). Number of neuron recorded, n = 12 from three DAT-iCre;Ai14;TbRII+/+ mice, and n = 13 from four DAT-iCre;Ai14;TbRIIfl/fl mice. Mann-Whitney test, ***p < 0.001. All data are presented as mean ± SEM.