Skip to main content
. 2016 Jul 6;7(37):58915–58930. doi: 10.18632/oncotarget.10410

Figure 3. Aloe-emodin suppressed oncoprotein YB-1 expression in HER-2-overexpressing breast cancer cells.

Figure 3

(A) SkBr3 cells were treated with various concentrations of AE for 48 h. Cell lysates were immunoblotted with anti-YB-1 antibody. β-Actin was used as the loading control. (B) SkBr3 cells were treated with various concentrations of AE for 48 h. HER-2 was visualized through immunofluorescence staining using anti-HER-2 antibodies (green). Labeling nuclear DNA using DAPI (blue). Scale bar, 40 μm. (C) SkBr3 cells were treated with various concentrations of AE for 24 h. The YB-1 mRNA levels were examined using real-time qPCR. Data are expressed in mean ± SD. (D) SkBr3 cells were treated with various concentrations of AE for 48 h and transfected with YBX-1-siRNA in combination with treatment with various concentrations of AE. Cell lysates were immunoblotted with anti-HER-2 and anti-YB-1 antibodies. β-Actin was used as the loading control.