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. 2016 Jul 6;7(37):58931–58938. doi: 10.18632/oncotarget.10431

Figure 4. lncRNA-CLMAT3 controlled the expression of master G0/G1 regulators, including Cdh1, CDKN1B (p27Kip1) and Skp2.

Figure 4

(A) Western blotting revealed the expression of Cdh1, p27Kip1, and Skp2 in LOVO cells. (B) The relative expression of Cdh1, p27Kip1, and Skp2 mRNA upon lncRNA-CLMAT3 knockdown was analyzed by real-time PCR. **P < 0.01 compared with control shRNA. (C) LOVO cell extracts were immunoprecipitated using antibodies against Skp2 or ubiquitin. Western blotting was used to detect interacting partners. (D) The blots were also probed with antibodies against Cdh1 to analyze interaction between Skp2 and Cdh1.