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. 2016 Jul 16;7(37):58995–59005. doi: 10.18632/oncotarget.10636

Figure 2. Mc alters SENP1 thermal stabilization.

Figure 2

(A–B). Four μg of purified SENP1C was incubated with 50 μM Mc at the indicated temperatures (A), and 4 μg purified SENP1C was incubated with indicated concentrations of Mc at 45°C for 3 min (B). After centrifugation, supernatant was analyzed by western blot with anti-SENP1 antibody and bands were scanned for densitometric analysis. The thermal melt curve (A) and the isothermal dose-response fingerprint (B) are shown. (C–D). Lysate from PC3 cells stably transfected with pBabe-Flag-SENP1 was treated with 100 μM Mc at the indicated temperatures (C) or with the indicated concentrations of Mc at 60°C for 3 min (D), then analyzed by western blot with anti-flag antibody. The bands were scanned for densitometric analysis, and the thermal melt curve (C) and the isothermal dose-response fingerprint (D) are shown.