Figure 2. Selection strategy for target genes of miR-101.
Analysis using the TargetScan program showed that 3,013 genes had putative target sites for miR-101 in their 3′ UTRs. Among these genes, 790 had conserved target sites among vertebrates for miR-101. Then, we assessed the expression levels of these genes in RCC clinical specimens using GEO expression profiles (GSE36985 and GSE22541). Finally, genes upregulated in RCC (log2 ratio > 1.0) were selected as putative target genes.