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. 2016 Jul 28;7(37):59070–59086. doi: 10.18632/oncotarget.10887

Figure 3. miR-101 directly downregulated UHRF1 expression in RCC cells.

Figure 3

(A) UHRF1 mRNA expression 72 h after transfection with miR-101. GUSB was used as an internal control. (B) UHRF1 protein expression 72 h after transfection with miR-101. GAPDH was used as a loading control. (C) miR-101 binding sites in UHRF1 mRNA. Luciferase reporter assays were carried out using a vector encoding the putative miR-101 target site in the UHRF1 3′-UTR (position 1030–1036) for wild-type and deletion constructs. *P < 0.002, **P < 0.0001. The bars indicate SDs.