Figure 4. Effects of miR-101 transfection on EZH2 mRNA and protein expression in RCC cells.

(A) EZH2 mRNA expression was determined at 72 h after transfection with miR-101. GUSB was used as an internal control. (B) EZH2 protein expression was evaluated by western blotting at 72 h after transfection with miR-101. GAPDH was used as a loading control. *P < 0.005. The bars indicate SDs.