Table 3. Sequencing characteristics of single-strand exome libraries.
Sample No. | Library ID | Mergetrimmed reads | Mapped reads on genome | % | Mapped reads on target | % | Unique reads | % | Mapped reads on target of unique | % | Molecules in library | Median molecule length (bp) | Mappable bp | Estimated exome coverage |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
22 | A8231 | 10400578 | 8880352 | 85 | 3664128 | 35 | 9027766 | 87 | 2694184 | 30 | 1.44E + 09 | 72 | 2.69E + 10 | 814 |
23 | A8232 | 13928657 | 8590615 | 62 | 4673607 | 34 | 7675101 | 55 | 4673607 | 61 | 1.02E + 09 | 57 | 1.95E + 10 | 591 |
24 | A8233 | 14725566 | 11548822 | 78 | 5440538 | 37 | 9148314 | 62 | 1400802 | 15 | 2.36E + 09 | 64 | 1.43E + 10 | 434 |
25 | A8234 | 13510755 | 9111893 | 67 | 4503396 | 33 | 7794873 | 58 | 445740 | 6 | 1.25E + 09 | 60 | 2.47E + 09 | 75 |
26 | A8235 | 13878538 | 9302472 | 67 | 4884491 | 35 | 7652137 | 55 | 434072 | 6 | 8.65E + 08 | 59 | 1.60E + 09 | 48 |
27 | A8236 | 12960033 | 9510227 | 73 | 4625240 | 36 | 7428921 | 57 | 652190 | 9 | 1.58E + 09 | 62 | 4.91E + 09 | 149 |
28 | A8237 | 14125480 | 8905778 | 63 | 4674789 | 33 | 8020581 | 57 | 344949 | 4 | 6.45E + 08 | 59 | 9.29E + 08 | 28 |
29 | A8238 | 13978045 | 8283185 | 59 | 4598067 | 33 | 7644855 | 55 | 190019 | 2 | 7.35E + 08 | 57 | 5.70E + 08 | 17 |
30 | A8239 | 12035341 | 8406977 | 70 | 3801180 | 32 | 8220549 | 68 | 1002280 | 12 | 2.84E + 09 | 71 | 1.68E + 10 | 509 |
31 | A8240 | 10933844 | 6549725 | 60 | 3043865 | 28 | 7709969 | 71 | 698527 | 9 | 2.72E + 09 | 71 | 1.23E + 10 | 374 |
32 | A8241 | 11869932 | 7089421 | 60 | 3557527 | 30 | 7483786 | 63 | 408108 | 5 | 1.58E + 09 | 65 | 3.52E + 09 | 107 |
33 | A8242 | 11869182 | 7085969 | 60 | 3872693 | 33 | 6586746 | 55 | 203156 | 3 | 2.54E + 09 | 55 | 2.39E + 09 | 72 |
NTC4 | A8243 | 1588390 | 394319 | 25 | 114244 | 7 | 1327044 | 84 | 10681 | 1 | 6.00E + 07 | n.d. | n.d. | n.d. |
14 | A8244 | 10484892 | 9184487 | 88 | 4079825 | 39 | 8752541 | 83 | 2839016 | 32 | 5.96E + 09 | 75 | 1.21E + 11 | 3668 |
15 | A8245 | 9956899 | 8806918 | 88 | 3742592 | 38 | 8234199 | 83 | 2480366 | 30 | 2.85E + 09 | 86 | 6.11E + 10 | 1850 |
16 | A8246 | 13621017 | 11778127 | 86 | 5246793 | 39 | 10605136 | 78 | 3062774 | 29 | 3.16E + 09 | 71 | 5.04E + 10 | 1529 |
NTC2 | A8247 | 1308857 | 295176 | 23 | 117051 | 9 | 1098888 | 84 | 8913 | 1 | 4.80E + 07 | n.d. | n.d. | n.d. |
N.d. not determined. NTC. Negative control.