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. 2016 Jul 8;7(37):59199–59208. doi: 10.18632/oncotarget.10498

Figure 4. Involvement of MEK/ERK signaling in nicotine-induced invasion/migration.

Figure 4

A. Induction of ERK phosphorylation by nicotine and the attenuation of the effect by α7-nAChR antagonism. B. Attenuation of nicotine-induced A549 cell invasion by MEK-ERK selective inhibitor U0126. * P < 0.05, ** P < 0.01 compared with control group; # P < 0.05 compared with nicotine alone group. C. Attenuation of nicotine-induced vimentin upregulation by MEK-ERK selective inhibitor U0126. The cells were treated by 3 μM nicotine for 30 min in western blot analysis and for 48 h in invasion and HCA assay; U0126 at 50 μM was added 5 minutes prior to the agonist. Quantifications in bar graphs are shown as means ± S.E.M from at least three independent experiments.