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. 2016 Aug 3;7(37):59245–59259. doi: 10.18632/oncotarget.11043

Figure 4. FOXM1 binds to human MET promoter and directly enhances its transcription.

Figure 4

(A) A putative FOXM1-binding site in the MET promoter and construction of reporter plasmids. (B) ChIP analysis of the MET promoter using antibodies against FOXM1 in PC9 and HCC827 cells. (C) The promoter activity of two truncated constructs was measured in PC9 and HCC827 cells when cotransfected with the control plasmid or FOXM1 shRNA plasmid. (D) The transcriptional activity of FOXM1 on MET-luc wide type (WT) or mutants (MT) was analyzed by luciferase reporter assay in PC9 and HCC827 cells. (E) The promoter activity of two truncated constructs was measured in PC9 and HCC827 cells when cotransfected with the control plasmid or pcDNA3.1-FOXM1 plasmid. Promoter activity was examined using a dual luciferase assay kit. The data represent three independent experiments, each bar represents mean ± SD. P values were calculated using Student t-test (**P < 0.01, ***P < 0.001, NS, nonsignificant).