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. 2016 Jul 29;7(37):59704–59713. doi: 10.18632/oncotarget.10923

Figure 1. Expression of MELOE-1 and MELOE-3 in melanoma cells.

Figure 1

(A) M113 melanoma cells were transfected with meloe-eGFP constructs: the native meloe cDNA (left panel), eGFP-MELOE-1 (middle panel) and eGFP-MELOE-3 (right panel). Analysis of fluorescent cells 48 h post-transfection was made using an automated fluorescence High Content Screening (HCS) microscopic device. Representative images out of 49 scanned fields per condition are shown. Nuclei were Hoescht stained prior to analysis. (B) Fluorescent eGFP-MELOE-1 (lane 2) or eGFP-MELOE-3 (lane 3) proteins were detected by western blot using an anti-eGFP mAb. Untransfected (UT) cells (lane 1) or cells transfected with the peGFP-N3 plasmid (lane 4) were used as controls. Amounts of total proteins loaded in each lane are indicated. A typical experiment is presented out of 4 performed.