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Melanocytes (01M10, 01M20), melanoma cell lines (M113, M117) and one colon carcinoma cell-line (SW707) were stained with a custom-made MELOE-3 monoclonal antibody (75 μg/mL) and a PE-coupled anti-mouse F(ab')2 (dark histograms) and analyzed by flow cytometry. A mouse control isotype was used as negative control (clear histograms).
