Figure 6. 3E10^EN/DOX-NPs have a significant effect on tumors.

A. Effect of nanoparticles on 4T1 tumor growth. Mice bearing 4T1 tumors of ~100 mm³ size were treated three times a week with intravenous injection of control PBS, free DOX, DOX-NPs, 3E10^EN-NPs, or 3E10^EN/DOX-NPs (n=7 mice per group). Tumor volumes were measured three times a week, and the resulting growth curves are shown. B. H&E staining of tumors with and without 3E10^EN/DOX-NP treatment. Tumors from control treatments revealed a highly cellular mass with prominent nuclei; in contrast, tumors from animals treated with 3E10^EN/DOX-NPs exhibited a much lower cellular mass and a lower nuclear-cytoplasmic ratio. C. TUNEL staining of tumors with and without nanoparticle treatment. Tumors in mice treated with 3E10^EN/DOX-NPs were found to have a marked increase in the number of apoptotic cells.