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. 2016 Aug 4;7(37):60074–60086. doi: 10.18632/oncotarget.11054

Figure 4. ST8SIA4 is targeted by miR-181c.

Figure 4

A. Predicted miR-181c target sequence in 3′UTRs of ST8SIA4 at two different sites. 293T cells were co-transfected with miR-181c or the control and luciferase reporter constructs or the mutants. MiR-181c repressed the activity of ST8SIA4-wt-3′UTR and ST8SIA4-wt1-3′UTR but had no effect on that of ST8SIA4-mut-3′UTR and ST8SIA4-mut1-3′UTR (*p<0.05). wt:ST8SIA4-wt-3′UTR(222-243), mut:ST8SIA4-mut-3′UTR(222-243), wt1:ST8SIA4-wt1-3′UTR(2117-2140), mut1:ST8SIA4- mut1-3′UTR(2117-2140). B. Western blotting of ST8SIA4 expression. GAPDH served as the loading control. C. The correlation between miR-181c and ST8SIA4 expression levels in CML/MDR (N = 22) and CML patients (N = 16)