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. 2016 Aug 4;7(37):60074–60086. doi: 10.18632/oncotarget.11054

Figure 6. Downregulation of miR-181c activated PI3K/AKT signaling.

Figure 6

A, B. Western blotting analysis of PI3K p110α, phosphorylated Akt (p-Akt 308), phosphorylated Akt (p-Akt 473), total Akt, and NF-κB protein levels in indicated cells. GAPDH was used as a loading control. C. Expression of PI3K/Akt signaling molecules were examined by western blot analysis, treated with Akt shRNA in K562/antagomiR-181c cells. D. Inactivation of PI3K/Akt signaling using Akt-shRNA significantly increased the chemo-sensitivity of K562 cells transfected with antagomiR-181c, analyzed by CCK-8 assay (*p<0.05).