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. 2016 Aug 11;7(37):60245–60269. doi: 10.18632/oncotarget.11197

Figure 5. PRIMA-1MET decreased expression of mutp53 and increased cleaved PARP-1 and GADD45A in GBM cells with MGMT knockdown.

Figure 5

Western blotting analysis of expression of MGMT and p53 (A) cleaved form of PARP-1 (89 kDa) (B) and GADD45A (C) in U87MG, A172, T98/EV and T98/shRNA GBM cell lines following 24-hour treatment with 40 μM (common dose) or the concentration corresponding to IC50 value of PRIMA-1MET in each cell line. Actin was used as a loading control. The density of the bands was normalized to that of DMSO controls (taken as 100%).