Table 2. Relative cell number and viable cells (%) in GBM cell lines treated with a range of PRIMA-1^MET doses.

PRIMA-1MET, μM	24 hours		48 hours		72 hours
	Cell number, %a	p-valueb	Cell number, %a	p-valueb	Cell number, %a	p-valueb
	T98/EV
0	107±9.99	-	100.2±2.3	-	100±5.03	-
25	71.2±5.25	0.0029	105±12.6	n.s.	121±11.5	0.03
50	67.6±19.7	0.0062	89.5±10.1	n.s.	106±9.56	n.s.
75	74.5±12.6	0.0097	89.5±12.1	n.s.	122±11.5	0.009
100	65.6±11.3	0.0017	78.9±11.5	n.s.	113.7±11.2	n.s.
	T98/shRNA
0	100±5.4	-	100±4.98	-	100±1.91	-
25	92.6±7.7	n.s.	82.5±12.8	0.02	63.9±11.7	< 0.0001
50	59.0±6.95	< 0.0001	44.9±11.4	< 0.0001	44.5±7.87	< 0.0001
75	45.2±11.4	< 0.0001	29.9±6.79	< 0.0001	20.2±1.97	< 0.0001
100	15.0±5.0	< 0.0001	26.3±10.7	< 0.0001	11.0±1.3	< 0.0001
	U138
0	100±1.8	-	100±10.7	-	100±10.4	-
25	75.1±7.1	0.01	82.4±1.05	0.017	72.3±6.02	< 0.0001
50	70.2±11.3	0.003	59.98±6.7	< 0.0001	63.1±10.7	< 0.0001
75	68.2±9.7	0.001	61.2±1.5	0.0002	52.9±3.4	< 0.0001
100	74.8±1.6	0.017	59.7±7.0	0.0002	40.9±3.1	< 0.0001
	LN-18
0	97±7.97	-	96.8±14.96	-	93.9±13.2	-
25	80.1±12.7	< 0.0001	98.5±13.7	n.s.	108.9±6.5	< 0.0001
50	96.4±12.6	n.s.	99.8±13.2	n.s.	93.1±7.99	n.s.
75	95.1±11.4	n.s.	75.3±14.3	< 0.0001	85.7±11.6	0.006
100	78.3±12.1	< 0.0001	72.5±10.6	< 0.0001	47.9±5.8	< 0.0001
	A172
0	100±8.2	-	100±8.6	-	100±3.7
25	79.95±8.6	0.002	83.9±6.8	0.004	72.2±5.6	< 0.0001
50	78.2±9.97	0.0004	69.4±5.8	< 0.0001	58.5±9.96	< 0.0001
75	65.3±5.9	< 0.0001	57.7±6.0	< 0.0001	67.3±4.5	< 0.0001
100	57.4±5.0	< 0.0001	51.4±8.6	< 0.0001	59.7±4.0	< 0.0001
	U87MG
0	100±2.8	-	98.2±4.1	-	100±5.0	-
25	89.8±2.1	0.001	71.95±7.2	< 0.0001	78.7±6.1	0.009
50	79.2±6.8	0.0005	40.5±4.3	< 0.0001	25.6±3.4	< 0.0001
75	60.7±7.7	< 0.0001	15.6±2.3	< 0.0001	12.97±10.79	< 0.0001
100	55.2 ±9.7	< 0.0001	18.7±2.0	< 0.0001	11.7±3.9	< 0.0001

PRIMA-1MET, μM	24 hours		48 hours		72 hours
	Viable cells,%a	p-valueb	Viable cells, %a	p-valueb	Viable cells, %a	p-valueb
	T98/EV
0	99±1.4	-	99.3±1.0	-	98.9±0.3	-
25	98.8±1.6	n.s.	99.8±0.5	n.s.	97.99±1.6	n.s.
50	98.3±2.4	n.s.	99±0.8	n.s.	98.7±1.2	n.s.
75	98.6±1.3	n.s.	99.5±0.6	n.s.	99.1±1.0	n.s.
100	99.6±0.9	n.s.	98.5±1.7	n.s.	98.1±1.9	n.s.
	T98/shRNA
0	99.5±1.0	-	98.4±0.9	-	98.5±0.8	-
25	98.5±1.7	n.s.	98±1.5	n.s.	99.2±0.98	n.s.
50	97±3.9	n.s.	97.3±1.9	n.s.	98.03±2.4	n.s.
75	69±11.3	< 0.0001	87.3±8.4	0.005	96.4±3.4	n.s.
100	7±3.6	< 0.0001	58.3±7.8	< 0.0001	81.8±5.02	< 0.0001
	U138
0	86.9±6.2	-	92.8±6.5	-	92.1±6.3	-
25	92.5±6.5	n.s.	88.8±0.4	n.s.	84.8±6.6	n.s.
50	73.3±5	0.001	77.6±9.6	0.01	81±4.3	n.s.
75	79.95±5	n.s.	84.6±0.7	n.s.	73.9±4.2	< 0.0001
100	67.5±1.9	0.001	25.2±3.7	< 0.0001	25.4±4.1	< 0.0001
	LN-18
0	96.3±6.2	-	95.1±5.2	-	95.7±5.4	-
25	96.8±6.6	n.s.	96.3±4.6	n.s.	98±3.1	n.s.
50	93.3±14.1	n.s.	96.9±3.2	n.s.	95.5±5.1	n.s.
75	95.2±12.9	n.s.	89.2±8	< 0.0001	96.7±4.3	n.s.
100	96.1±7.1	n.s.	90.3±8.6	0.0007	88.5±10.6	< 0.0001
	A172
0	98.3±1.4	-	97.5±1.6	-	98.3±0.9	-
25	98±0.6	n.s.	99.4±0.8	n.s.	97.2±1.3	n.s.
50	97.1±2.2	n.s.	93.6±3.8	n.s.	91.8±3.6	0.031
75	95.7±2.5	n.s.	86.9±7.2	0.0055	83.9±6.9	< 0.0001
100	93.7±5.3	0.027	71.7±7.3	< 0.0001	73.6±5.7	< 0.0001
	U87MG
0	98.4±1.5	-	96.9±2	-	96.8±3.3	-
25	98.4±1.3	n.s.	97.1±3.6	n.s.	95.4±4.1	n.s.
50	99.03±0.8	n.s.	93.8±6.5	n.s.	90.4±0.2	n.s.
75	96.1±6.1	n.s.	84.5±7.4	0.0027	78.1±11.3	0.0005
100	96.8±5.3	n.s.	9.2±2.0	< 0.0001	13.7±10.5	< 0.0001

^aMean ± SD

^bCompared to DMSO control at the corresponding time point

n.s. – not significant