Table 2. Relative cell number and viable cells (%) in GBM cell lines treated with a range of PRIMA-1MET doses.
PRIMA-1MET, μM | 24 hours | 48 hours | 72 hours | |||
---|---|---|---|---|---|---|
Cell number, %a | p-valueb | Cell number, %a | p-valueb | Cell number, %a | p-valueb | |
T98/EV | ||||||
0 | 107±9.99 | - | 100.2±2.3 | - | 100±5.03 | - |
25 | 71.2±5.25 | 0.0029 | 105±12.6 | n.s. | 121±11.5 | 0.03 |
50 | 67.6±19.7 | 0.0062 | 89.5±10.1 | n.s. | 106±9.56 | n.s. |
75 | 74.5±12.6 | 0.0097 | 89.5±12.1 | n.s. | 122±11.5 | 0.009 |
100 | 65.6±11.3 | 0.0017 | 78.9±11.5 | n.s. | 113.7±11.2 | n.s. |
T98/shRNA | ||||||
0 | 100±5.4 | - | 100±4.98 | - | 100±1.91 | - |
25 | 92.6±7.7 | n.s. | 82.5±12.8 | 0.02 | 63.9±11.7 | < 0.0001 |
50 | 59.0±6.95 | < 0.0001 | 44.9±11.4 | < 0.0001 | 44.5±7.87 | < 0.0001 |
75 | 45.2±11.4 | < 0.0001 | 29.9±6.79 | < 0.0001 | 20.2±1.97 | < 0.0001 |
100 | 15.0±5.0 | < 0.0001 | 26.3±10.7 | < 0.0001 | 11.0±1.3 | < 0.0001 |
U138 | ||||||
0 | 100±1.8 | - | 100±10.7 | - | 100±10.4 | - |
25 | 75.1±7.1 | 0.01 | 82.4±1.05 | 0.017 | 72.3±6.02 | < 0.0001 |
50 | 70.2±11.3 | 0.003 | 59.98±6.7 | < 0.0001 | 63.1±10.7 | < 0.0001 |
75 | 68.2±9.7 | 0.001 | 61.2±1.5 | 0.0002 | 52.9±3.4 | < 0.0001 |
100 | 74.8±1.6 | 0.017 | 59.7±7.0 | 0.0002 | 40.9±3.1 | < 0.0001 |
LN-18 | ||||||
0 | 97±7.97 | - | 96.8±14.96 | - | 93.9±13.2 | - |
25 | 80.1±12.7 | < 0.0001 | 98.5±13.7 | n.s. | 108.9±6.5 | < 0.0001 |
50 | 96.4±12.6 | n.s. | 99.8±13.2 | n.s. | 93.1±7.99 | n.s. |
75 | 95.1±11.4 | n.s. | 75.3±14.3 | < 0.0001 | 85.7±11.6 | 0.006 |
100 | 78.3±12.1 | < 0.0001 | 72.5±10.6 | < 0.0001 | 47.9±5.8 | < 0.0001 |
A172 | ||||||
0 | 100±8.2 | - | 100±8.6 | - | 100±3.7 | |
25 | 79.95±8.6 | 0.002 | 83.9±6.8 | 0.004 | 72.2±5.6 | < 0.0001 |
50 | 78.2±9.97 | 0.0004 | 69.4±5.8 | < 0.0001 | 58.5±9.96 | < 0.0001 |
75 | 65.3±5.9 | < 0.0001 | 57.7±6.0 | < 0.0001 | 67.3±4.5 | < 0.0001 |
100 | 57.4±5.0 | < 0.0001 | 51.4±8.6 | < 0.0001 | 59.7±4.0 | < 0.0001 |
U87MG | ||||||
0 | 100±2.8 | - | 98.2±4.1 | - | 100±5.0 | - |
25 | 89.8±2.1 | 0.001 | 71.95±7.2 | < 0.0001 | 78.7±6.1 | 0.009 |
50 | 79.2±6.8 | 0.0005 | 40.5±4.3 | < 0.0001 | 25.6±3.4 | < 0.0001 |
75 | 60.7±7.7 | < 0.0001 | 15.6±2.3 | < 0.0001 | 12.97±10.79 | < 0.0001 |
100 | 55.2 ±9.7 | < 0.0001 | 18.7±2.0 | < 0.0001 | 11.7±3.9 | < 0.0001 |
PRIMA-1MET, μM | 24 hours | 48 hours | 72 hours | |||
---|---|---|---|---|---|---|
Viable cells,%a | p-valueb | Viable cells, %a | p-valueb | Viable cells, %a | p-valueb | |
T98/EV | ||||||
0 | 99±1.4 | - | 99.3±1.0 | - | 98.9±0.3 | - |
25 | 98.8±1.6 | n.s. | 99.8±0.5 | n.s. | 97.99±1.6 | n.s. |
50 | 98.3±2.4 | n.s. | 99±0.8 | n.s. | 98.7±1.2 | n.s. |
75 | 98.6±1.3 | n.s. | 99.5±0.6 | n.s. | 99.1±1.0 | n.s. |
100 | 99.6±0.9 | n.s. | 98.5±1.7 | n.s. | 98.1±1.9 | n.s. |
T98/shRNA | ||||||
0 | 99.5±1.0 | - | 98.4±0.9 | - | 98.5±0.8 | - |
25 | 98.5±1.7 | n.s. | 98±1.5 | n.s. | 99.2±0.98 | n.s. |
50 | 97±3.9 | n.s. | 97.3±1.9 | n.s. | 98.03±2.4 | n.s. |
75 | 69±11.3 | < 0.0001 | 87.3±8.4 | 0.005 | 96.4±3.4 | n.s. |
100 | 7±3.6 | < 0.0001 | 58.3±7.8 | < 0.0001 | 81.8±5.02 | < 0.0001 |
U138 | ||||||
0 | 86.9±6.2 | - | 92.8±6.5 | - | 92.1±6.3 | - |
25 | 92.5±6.5 | n.s. | 88.8±0.4 | n.s. | 84.8±6.6 | n.s. |
50 | 73.3±5 | 0.001 | 77.6±9.6 | 0.01 | 81±4.3 | n.s. |
75 | 79.95±5 | n.s. | 84.6±0.7 | n.s. | 73.9±4.2 | < 0.0001 |
100 | 67.5±1.9 | 0.001 | 25.2±3.7 | < 0.0001 | 25.4±4.1 | < 0.0001 |
LN-18 | ||||||
0 | 96.3±6.2 | - | 95.1±5.2 | - | 95.7±5.4 | - |
25 | 96.8±6.6 | n.s. | 96.3±4.6 | n.s. | 98±3.1 | n.s. |
50 | 93.3±14.1 | n.s. | 96.9±3.2 | n.s. | 95.5±5.1 | n.s. |
75 | 95.2±12.9 | n.s. | 89.2±8 | < 0.0001 | 96.7±4.3 | n.s. |
100 | 96.1±7.1 | n.s. | 90.3±8.6 | 0.0007 | 88.5±10.6 | < 0.0001 |
A172 | ||||||
0 | 98.3±1.4 | - | 97.5±1.6 | - | 98.3±0.9 | - |
25 | 98±0.6 | n.s. | 99.4±0.8 | n.s. | 97.2±1.3 | n.s. |
50 | 97.1±2.2 | n.s. | 93.6±3.8 | n.s. | 91.8±3.6 | 0.031 |
75 | 95.7±2.5 | n.s. | 86.9±7.2 | 0.0055 | 83.9±6.9 | < 0.0001 |
100 | 93.7±5.3 | 0.027 | 71.7±7.3 | < 0.0001 | 73.6±5.7 | < 0.0001 |
U87MG | ||||||
0 | 98.4±1.5 | - | 96.9±2 | - | 96.8±3.3 | - |
25 | 98.4±1.3 | n.s. | 97.1±3.6 | n.s. | 95.4±4.1 | n.s. |
50 | 99.03±0.8 | n.s. | 93.8±6.5 | n.s. | 90.4±0.2 | n.s. |
75 | 96.1±6.1 | n.s. | 84.5±7.4 | 0.0027 | 78.1±11.3 | 0.0005 |
100 | 96.8±5.3 | n.s. | 9.2±2.0 | < 0.0001 | 13.7±10.5 | < 0.0001 |
Mean ± SD
Compared to DMSO control at the corresponding time point
n.s. – not significant