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. 2016 Aug 2;7(37):60407–60418. doi: 10.18632/oncotarget.11004

Figure 2. Regulation of AR by Lin28A is mediated by regulation of c-myc.

Figure 2

A. MDA-MB-453 cells were cotransfected with control or Lin28AsiRNA along with the pGL4-AR-Prom-Luc reporter. AR promoter activity was repressed when Lin28A was down-regulated. Data points represent the mean±S.D. of triplicate samples from two independent experiments. RLU: Relative Luciferase Units. B. SK-BR-3 cells were cotransfected with control or Lin28A along with the pGL4-AR-Prom-Luc reporter. AR promoter activity was enhanced in Lin28A-overexpressing cells. C. MDA-MB-453 cells were cotransfected with control or c-myc shRNA along with the pGL4-AR-Prom-Luc reporter. Luciferase assays showed that down-regulation of c-myc reduced transactivation of the AR promoter. D. SK-BR-3 cells were cotransfected with control or c-myc along with the pGL4-AR-Prom-Luc reporter. Overexpression of c-myc enhanced AR promoter activity. E. SK-BR-3 cells were transfected with Lin28A alone or Lin28A and c-myc shRNA together along with the pGL4-AR prom-Luc reporter. Repression of c-myc could overcome the promotion of AR promoter activity by Lin28A. F, G. Recruitment of c-myc to the c-myc binding site in the AR promoter was analyzed by ChIP assays. Overexpression of Lin28A enhanced the recruitment of c-myc to the AR promoter. Down-regulation of Lin28A reduced the recruitment of c-myc to the AR promoter.