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. 2016 Aug 5;7(37):60432–60445. doi: 10.18632/oncotarget.11080

Figure 3. MiR-142-3p targets the Bod1 3′-UTR and suppresses its expression.

Figure 3

A. The putative miR-142-3p binding site in the human Bod1 3′-UTR is shown at the top. The mature miR-142-3p sequence is shown aligned to the target site, and the mutated miR-142-3p seed-pairing sequence is shown below. B. Luciferase reporter assays were performed 24 h after 786-O cells were co-transfected with Wt Bod1 or Mut Bod1 vectors together with miR-142-3p mimics or nonsense small RNA oligonucleotides as the negative control. C. Relative Bod1 mRNA expression was measured by qRT-PCR after 786-O and A549 cells were transfected with miR-142-3p or negative control (NC). U6 and GAPDH were used as internal controls. D. Bod1 protein levels were measured in 786-O in A549 cells by western blot after transfection with miR-142-3p or negative control (NC). Each data point represents the mean of three separate experiments; bars indicate standard errors. **P < 0.01.