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Barx2 mRNA levels a. and protein levels b. in gastric mucosa cell line (GES-1) and 7 GC cell lines. Barx2 mRNA and protein expression in BGC-823 and SGC-7901 cells transfected with Barx2 overexpression or downregulation vectors were validated using quantitative real-time PCR c. and Western blotting d. GAPDH was used to normalize mRNA and protein levels.
