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Phase contrast images of HepG-2 cells transfected with either siControl or siTC4 A. Differentiation marker expression was analyzed by western blotting B. or IF C. Cell proliferation was assessed by Ki-67 IF staining D., proliferation assay E. and colony formation assay F. ShTCF4- or shControl-transfected cells were injected into NOD/SCID mice subcutaneously and tumor volumes were recorded G. *P<0.05 vs. control.
