Coverage of the (a) apolar, and (b) polar lipid classes by commonly used separation methods. RP, reversed phase; ACN, acetonitrile; IPA, isopropanol; NP, normal phase; HILIC, hydrophilic interaction liquid chromatography; CE, cholesterol ester; TG, triglyceride; (L)SM, (lyso)sphingomyelin; DG, diglyceride; (L)PC, (lyso)phosphatidylcholine; PE, phosphatidylethanolamine; (L)PS, (lyso)phosphatidylserine; (L)PG, (lyso)phosphatidylglycerol; (L)PI, (lyso)phosphatidylinositol; Cer, ceramide; MG, monoglyceride; So, sphingosine; S1P, sphingosine-1-phosphate; FA, fatty acid; AcylCN, acyl-carnitine.