Fig. 7.

First trimester (FTM) and term human umbilical cord perivascular cells (HUCPVCs) generate haploid cells. a Representative flow cytometry plots for cell cycle analysis of diploid undifferentiated (UNDIFF) HUCPVCs (showing FTM HUCPVCs, left panel), haploid spermatozoa (SPERM, second panel) and FTM HUCPVCs and term HUCPVCs at the end of Step 5 (right panels, respectively). b Quantification of the percentage of haploid cells in FTM HUCPVCs and term HUCPVCs, comparing undifferentiated HUCPVCs with HUCPVCs at the end of Step 4 and Step 5. Each condition was analyzed in three to four independent experiments. *p < 0.05. c Representative micrographs of FTM HUCPVCs processed for fluorescence in-situ hybridization (FISH) at Step 5, showing an example of a diploid cell observed at Step 5 displaying chromosome X (red), Y (green) and 2 chromosome 18 (yellow) and an example of a haploid cells at Step 5 displaying chromosome Y (green) and one chromosome 18 (yellow)