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. 2017 Feb 1;23(3-4):101–114. doi: 10.1089/ten.tea.2016.0007

FIG. 2.

FIG. 2.

(A) Relative gene expression by NCs cocultured with AMs as assessed by qRT-PCR. RPL-32 served as the reference gene, and the data are normalized to the NC mono-culture control. (B) Protein level production of Col-2 and aggrecan by NCs cocultured with AMs relative to NCs in mono-culture as assessed by semi-quantitative immunostaining. (C) Relative protein expression of the transcription factor Sox-9 and various MMPs and cytokines as assessed by western blot or multiplex assays. Data are normalized to the DNA content in each sample and further normalized to the NC mono-culture control. In each panel, significant differences between the coculture and mono-culture groups are indicated either by an asterisk (p < 0.05) or a pound symbol (p < 0.01). At least three samples per culture group were analyzed per assessment technique. qRT-PCR, quantitative reverse transcription polymerase chain reaction.