Figure 2.

AZA and DAC cause DNMT1 depletion in NSCLC cell lines. A549 and H1299 cells were treated with AZA or DAC (0–5 µM) for 20 hours and DNMT1 protein was detected by Western blotting of cell extracts. Alpha-tubulin was used as a loading control.

Abbreviations: AZA, azacitidine; DAC, decitabine; DNMT1, DNA methyltransferase 1; NSCLC, non-small cell lung cancer.