Fig. 2.

Lipoplexes (A) and liposomes (B) lead to TNF-α secretion in human macrophages. Primed THP1 cells were incubated for 5 h with the indicate amount of cationic lipids (μM) in serum free medium, then cell supernatants were collected and TNF-α was quantified using ELISA assay following manufacturer's instructions. Each bar represents the mean + standard deviation of three replicate values (n = 3) after subtraction of the secretion measured in absence of stimulant. The experiment is representative of at least 3 independent replicates.