Figure 1.

AAV7m8 targets the non-human primate (NHP) outer and inner retina efficiently by subretinal delivery. A cartoon of the adeno-associated virus (AAV) vector is shown comprising a hybrid CMV enhancer-CBA promoter driving eGFP expression (a). Confocal images of retinal sections expressing eGFP (green) post injection with various doses of AAV7m8-CMV/CBA-eGFP: 1E + 10 vg (b, c, and d), 1E + 11 vg (e, f, and g), and 1E + 12 vg (h, i, and j). Co-localization of eGFP with PNA (red), a marker of cone cells, in shown in (c), (f), and (i). The fovea is represented in (k) with eGFP expressed in the ganglion cells. DAPI stained nuclei are shown in magenta. An overlay of DAPI-stained sections is included on the far left of panels (c), (f), and (i) in order to indicate the cell layers. The layer including DAPI fluorescence is eliminated from the remainder of those panels, as it obscures eGFP-positive cells in the nuclear layers. OS/IS, outer segment, inner segment; ONL, outer nuclear layer; INL, inner nuclear layer, GCL, ganglion cell layer. Scale bars represent 50 μm. Color images available online at www.liebertpub.com/hum