Figure 3.

AAV8BP2 transduces photoreceptors efficiently in the primate retina by subretinal delivery. At all three doses 1E + 10 vg (a, b, and c), 1E + 11 vg (d, e, f), and 1E + 12 vg (g, h, and i), AAV8BP2 transduced the photoreceptors (in particular the cones), RPE cells, and optic nerve fibers (c, f, and i). The tissue in panel (c) was oriented tangential to that shown in (f and i). Co-localization of eGFP in the cone outer segments is seen with PNA staining (red) of the cones in the central panel (b, e, and h). An overlay of DAPI-stained sections is included on the far left of panels (b), (e), and (h) in order to indicate the cell layers. Very few bipolar cells were weakly eGFP positive. eGFP fluorescence is seen in green, and DAPI stained nuclei are shown in magenta. Scale bars represent 50 μm. Color images available online at www.liebertpub.com/hum