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Effect of Nicotine on 5-mC and DNMT Expression
EBs exposed to 10 μM nicotine with or without 10 μM Hexa were subjected to 12-day differentiation.
(A and B) 5-Methylcytosine (5-mC) was tested by dot blot and ELISA assay. Methylene blue (MB) staining of the membranes was performed to confirm equal DNA loading.
(C and D) Western blot analysis and quantification of DNMT1 and DNMT3A.
Data represent the mean ± SEM of three independent experiments. ∗∗p < 0.01, #p < 0.001.
