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. 2017 Jan 19;8(2):290–304. doi: 10.1016/j.stemcr.2016.12.016

Figure 4.

Figure 4

Methylation Status of Tbx5 and Gata4 Promoter Modulated by Nicotine in 12-Day Differentiated EBs

EBs exposed to 10 μM nicotine with or without 10 μM Hexa were subjected to 12-day differentiation.

(A and D) Schematic representing the distribution of CpG sites in mouse Tbx5 (A) and Gata4 (D) genes with the selected CpG island of a 190-bp fragment (−95 bp to +95 bp) and a 278-bp fragment (−157 bp to +121 bp) within the Tbx5 and Gata4 promoter region, respectively. Transcription start site is indicated by a curved arrow. Each vertical bar represents a single CpG site.

(B and E) Methylation status of selected CpG loci within mouse Tbx5 (B) and Gata4 (E) gene promoter regions.

(C and F) BSP sequencing results of selected CpG loci within the mouse Tbx5 (C) and Gata4 (F) gene promoter region.

Ten colonies of PCR products from each bisulfite-treated DNA sample are sequenced and each is shown as an individual row. One circle indicates one CpG site, and closed or open circles represent methylated or unmethylated cytosines, respectively. Data represent the mean ± SEM of three independent experiments. ∗p < 0.05, ∗∗p < 0.01.