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. 2017 Jan 19;8(2):290–304. doi: 10.1016/j.stemcr.2016.12.016

Figure 6.

Figure 6

Methylation Status of Tbx5 and Gata4 Promoter in Offspring Heart with Maternal Exposure to Nicotine and nAChR Antagonist

Pregnant rats were subjected to 50 μg/mL nicotine or 100 μg/mL nicotine with or without 100 μg/mL Hexa exposure during gestation. Methylation status of Tbx5 and Gata4 promoter in offspring heart was tested.

(A and D) Schematic representing the distribution of CpG sites in rat Tbx5 (A) and Gata4 (D) genes with the selected CpG island of a 247-bp fragment (−340 bp to −93 bp) and a 264-bp fragment (−165 bp to 99 bp) within the Tbx5 and Gata4 promoter region, respectively. Transcription start site is indicated by a curved arrow. Each vertical bar represents a single CpG site.

(B and E) Methylation status of selected CpG loci within the rat Tbx5 (B) and Gata4 (E) gene promoter region.

(C and F) BSP sequencing results of selected CpG loci within the rat Tbx5 (C) and Gata4 (F) gene promoter region.

Ten colonies of PCR products from each bisulfite-treated DNA sample are sequenced and each is shown as an individual row with open circles for unmethylated and filled circles for methylated CpG islands. Data represent the mean ± SEM of three independent experiments. ∗p < 0.05, ∗∗p < 0.01.