FIG. 2.

Soluble factors secreted by EC can induce latent HIV infections in resting CD4⁺ T cells. Resting CD4⁺ T cells were cultured with or without EC in transwells and infected 1 day later with a GFP reporter virus. GFP-negative cells were sorted on day 8 postinfection and treated with or without PMA/I to reactivate latent virus (labeled as induced and uninduced, respectively). GFP was measured 2 days after PMA/I treatment. Samples were taken in duplicates, and means ± standard errors are plotted. Data shown are the representative of three independent experiments yielding similar results. *Student t-test; p < .05. ^#Uninduced resting T cells at 0%. PMA, phorbol myristate acetate; PMA/I, PMA and Ionomoycin.