FIG. 3.

Early removal of T cells from EC resulted in reduced productive and latent infections. (A) Early removal of resting CD4⁺ T cells stimulated by direct contact with EC. Resting T cells were cultured in contact with EC⁻ or EC⁺, infected with a GFP reporter virus, and were removed at various time intervals after infection. The %GFP⁺ cells were measured on day 6 postinfection for all cultures, and the days that the resting CD4⁺ T cells were removed postinfection are as indicated. Samples were taken in triplicates, and means ± standard errors are plotted. Data shown are the representative of three independent experiments yielding similar results. (B) Early removal of resting CD4⁺ T cells stimulated by soluble factors with EC. Resting CD4⁺ T cells were cultured with EC⁻ or EC⁺ in transwells, infected 1 day later with a GFP reporter virus, and removed at the indicated days postinfection. GFP expressions were measured on day 7 after infection. Samples were taken in quadruplicates, and means ± standard errors are plotted. Data shown are the representative of four independent experiments yielding similar results. (C) Latent infection in early removal of resting CD4⁺ T cells from EC stimulation. Resting CD4⁺ T cells were cultured in direct contact with EC, and on various days postinfection, a portion of cells were removed. On day 8 postinfection, GFP-negative cells were sorted and cultured with or without PMA/I (labeled as induced and uninduced, respectively) for 2 days to reactivate latent virus. Samples were taken in triplicates, and means ± standard errors are plotted. Data shown are the representative of three independent experiments yielding similar results. *Student t-test; p < .05.