FIG. 1.

Antcin H protects against liver injury induced by APAP. (A, B) Mice received 25 and 50 mg/kg Antcin H i.p. in corn oil 1 h before APAP (300 mg/kg), and serum ALT and representative histology (H&E) determined at 24 h. ***p < 0.001, n = 3–4 mice per group. (C, D) Antcin H (50 mg/kg) was administered 1 h after APAP (300 mg/kg), and serum ALT and histology determined at 24 h. **p < 0.01, n = 6 mice per group. (E) Cultured PMH were pretreated with 50 and 75 μM Antcin H for 2 h and then switched to APAP (10 mM) plus Antcin H. Necrotic cells (SYTOX Green positive) were counted in 10 different fields per dish at 24 h. *p < 0.05 (n = 3 experiments). ALT, alanine aminotransferase; APAP, acetaminophen; H&E, hematoxylin and eosin. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars