FIG. 2.

Antcin H protects against the liver injury induced by GalN/TNFα. (A, B) Mice were pretreated with Antcin H (25, 50 mg/kg in DMSO) twice, 6 and 1 h, before GalN (800 mg/kg)/TNFα (12 μg/kg) i.p. After 6 h, serum ALT level and liver histology (H&E) were determined ***p < 0.001(n = 3 mice per group). (C) Cultured PMH were treated with different concentrations of Antcin H (50, 75, and 100 μM) and Act D (0.5 μg/ml)/TNFα (5 ng/ml) simultaneously. After 6 h, percent apoptosis was determined (Hoechst 33248). *p < 0.05, **p < 0.01 (n = 3 experiments). GalN, galactosamine; TNF, tumor necrosis factor. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars